Fixtures
| Day | Date | Home | V | Away | Kick-Off |
| Sat | 08-Aug-09 | Stevenage Borough | 1-1 | Tamworth | 3.00pm |
| Tue | 11-Aug-09 | Hayes & Yeading | 1-1 | Stevenage Borough | 7.45pm |
| Sat | 15-Aug-09 | Barrow | 0-0 | Stevenage Borough | 3.00pm |
| Tue | 18-Aug-09 | Stevenage Borough | 3-0 | Ebbsfleet United | 7.45pm |
| Sat | 22-Aug-09 | Oxford United | 2-1 | Stevenage Borough | 3.00pm |
| Sat | 29-Aug-09 | Stevenage Borough | 2-1 | Rushden & Diamonds | 3.00pm |
| Tue | 31-Aug-09 | Histon | 0-2 | Stevenage Borough | 7.45pm |
| Tue | 08-Sep-09 | Stevenage Borough | 2-0 | Eastbourne Borough | 7.45pm |
| Sat | 12-Sep-09 | Mansfield Town | 2-3 | Stevenage Borough | 3.00pm |
| Sat | 19-Sep-09 | Chester City | 0-1 | Stevenage Borough | 3.00pm |
| Tue | 22-Sep-09 | Stevenage Borough | 1-1 | Grays Athletic | 7.45pm |
| Sat | 26-Sep-09 | Stevenage Borough | 1-1 | Altrincham | 3.00pm |
| Tue | 29-Sep-09 | Luton Town | 0-1 | Stevenage Borough | 7.45pm |
| Sat | 03-Oct-09 | York City | 1-1 | Stevenage Borough | 3.00pm |
| Sat | 10-Oct-09 | Stevenage Borough | 4-0 | Hayes & Yeading | 3.00pm |
| Sat | 17-Oct-09 | Stevenage Borough | 3-1 | Salisbury City | 3.00pm |
| Tue | 20-Oct-09 | Stevenage Borough | 0-0 | Wrexham | 7.45pm |
| Sat | 31-Oct-09 | Kettering Town | 1-1 | Stevenage Borough | 3.00pm |
| Sat | 14-Nov-09 | Stevenage Borough | - | Gateshead | 3.00pm |
| Sat | 21-Nov-09 | Forest Green Rovers | - | Stevenage Borough | 3.00pm |
| Tue | 24-Nov-09 | Stevenage Borough | - | Chester City | 7.45pm |
| Sat | 28-Nov-09 | Salisbury City | - | Stevenage Borough | 3.00pm |
| Tue | 01-Dec-09 | Ebbsfleet United | - | Stevenage Borough | 7.45pm |
| Sat | 05-Dec-09 | Stevenage Borough | - | Kidderminster Harriers | 3.00pm |
| Sat | 19-Dec-09 | Stevenage Borough | - | Barrow | 3.00pm |
| Sat | 26-Dec-09 | Cambridge United | - | Stevenage Borough | 3.00pm |
| Mon | 28-Dec-09 | Stevenage Borough | - | AFC Wimbledon | 7.45pm |
| Fri | 01-Jan-10 | Stevenage Borough | - | Cambridge United | 3.00pm |
| Sat | 16-Jan-10 | Wrexham | - | Stevenage Borough | 3.00pm |
| Tue | 19-Jan-10 | Eastbourne Borough | - | Stevenage Borough | 7.45pm |
| Sat | 23-Jan-10 | Stevenage Borough | - | Kettering Town | 3.00pm |
| Sat | 30-Jan-10 | Altrincham | - | Stevenage Borough | 3.00pm |
| Sat | 06-Feb-10 | Tamworth | - | Stevenage Borough | 3.00pm |
| Tue | 09-Feb-10 | Stevenage Borough | - | Mansfield Town | 7.45pm |
| Sat | 13-Feb-10 | Rushden & Diamonds | - | Stevenage Borough | 3.00pm |
| Sat | 20-Feb-10 | Stevenage Borough | - | Oxford United | 3.00pm |
| Sat | 06-Mar-10 | Stevenage Borough | - | Crawley Town | 3.00pm |
| Sat | 13-Mar-10 | Grays Athletic | - | Stevenage Borough | 3.00pm |
| Tue | 16-Mar-10 | Stevenage Borough | - | Histon | 7.45pm |
| Sat | 20-Mar-10 | Gateshead | - | Stevenage Borough | 3.00pm |
| Sat | 27-Mar-10 | Crawley Town | - | Stevenage Borough | 3.00pm |
| Sat | 03-Apr-10 | Stevenage Borough | - | Luton Town | 3.00pm |
| Mon | 05-Apr-10 | AFC Wimbledon | - | Stevenage Borough | 3.00pm |
| Sat | 10-Apr-10 | Stevenage Borough | - | Forest Green Rovers | 3.00pm |
| Sat | 17-Apr-10 | Kidderminster Harriers | - | Stevenage Borough | 3.00pm |
| Sat | 24-Apr-10 | Stevenage Borough | - | York City | 3.00pm |
Pre Season
| Day | Date | Home | V | Away | Kick-Off |
| Wed | 15-Jul-09 | Chelmsford City | 1-2 | Stevenage Borough | 7.45pm |
| Sat | 18-Jul-09 | Stevenage Borough | 2-1 | Brighton | 3.00pm |
| Tue | 21-Jul-09 | Cambridge City | 1-1 | Stevenage Borough | 7.45pm |
| Sat | 25-Jul-09 | Stevenage Borough | 1-2 | Millwall | 3.00pm |
| Tue | 28-Jul-09 | Stevenage Borough | 0-2 | Peterborough United | 7.45pm |
| Fri | 31-Jul-09 | Stevenage Borough | 2-1 | Southend United | 7.45pm |
| Sat | 01-Aug-09 | Woking | 0-0 | Stevenage Borough | 3.00pm |
